Article 9119

Title of the article



Dudina Marina Olegovna, Applicant, National Research Mordovia State University (68 Bolshevistskaya street, Saransk, Russia), E-mail:
Blinova Ekaterina Valerievna, Doctor of medical sciences, associate professor, professor, sub-department of faculty surgery, head of laboratory specific pharmacological activities of the Center for Advanced Studies of innovative medicines, National Research Mordovia State University (68 Bolshevistskaya street, Saransk, Russia), E-mail:
Moiseeva Inessa Yakovlevna, Doctor of medical sciences, professor, head of the sub-department of general and clinical pharmacology, dean of the faculty of medicine, Penza State University (40 Krasnaya street, Penza, Russia), E-mail:
Samyshina Elena Aleksandrovna, Candidate of medical sciences, senior staff scientist, of laboratory specific pharmacological activities of the Center for Advanced Studies of innovative medicines, National Research Mordovia State University (68 Bolshevistskaya street, Saransk, Russia), E-mail:
Krasko Mariya Olegovna, Postgraduate student, National Research Mordovia State University (68 Bolshevistskaya street, Saransk, Russia), E-mail:
Suslova Irina Rudol'fovna, Applicant, sub-department of faculty surgery, National Research Mordovia State University (68 Bolshevistskaya street, Saransk, Russia), E-mail: 

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Background. To study the acute toxicity of compound AH-554 after intragastric administration, as well as to determine its effective cytotoxic concentration and dose.
Materials and methods. The study was performed on 76 white outbred laboratory mice of both sexes weighing 18–22 g, 150 C57Bl / 6 male mice, and LCC tumor cell culture. The acute toxicity of 4-alkyl-substituted compound AH-554 as granulate tablet mass was studied after intragastric administration by Lichfield and Wilcoxon. The substance effective dose was determined in C57Bl/6 male mice syngeneic tumor model with inoculated Lewis lung carcinoma. AH-554 effective concentration was evaluated in the tumor cell culture.
Results. The study of acute toxicity of AH-554 as granulate of tablet mass administered intragastrically showed that the compound is non-toxic. In the dose range from 21.2 to 384 mg / kg, the substance inhibits tumor growth in mice with syngeneic lung carcinoma from 20 to 90%, while the highest therapeutic dose exceeds the minimum effective more than 18 times. The resulting pattern is the same when cultivating the substance in the culture of tumor cells. The results of the study can be used to create novel medicine based on the compound AH-554.
Conclusions. The 2-amino-4H-chromene AH-554 derivative has an optimal safety profile, since it is a non-toxic substance with a large range of antitumor action. 

Key words

2-amino-4H-chromene AH-554 derivative, acute toxicity, route of administration, range of therapeutic action, Lewis lung carcinoma 

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Дата обновления: 26.06.2019 13:51